Molecular Test Listing

Molecular Requisition

Prothrombin (Factor II) is the second most common genetic predisposition risk to venous thromboembolisms. Venous thrombosis is a worldwide health problem resulting in significant illness and death. Both genetic as well as environmental factors, e.g. smoking and oral contraceptives, contribute to the incidence of the disease. Individuals that carry one copy of the 20210G>A mutation in the prothrombin gene are at a 3- to 8-fold increased risk for venous thrombosis with a further increase in individuals that carry two of these mutations. The mutation has an overall prevalence of approximately 2% in the Caucasian population but is significantly lower in other ethnic groups.

• To determine the potential cause of unexplained thrombosis in children or adults.
• Patients undergoing surgery especially the potentially high-risk class of women taking estrogen supplements.
• Evaluation of individuals before initiation of oral contraceptives.

• Testing may be combined with testing for FactorV, MTHFR, & HPA1 in a Thrombosis Risk Panel.

• PCR amplification of the region including the G1691A mutation, followed by mutation specific restriction enzyme digestion.

• Blood EDTA (purple top) tubes:  

            Newborn: minimum 1-2 mL          

            Child/Adult: prefer 2 tubes of 3-5 mL

• Prenatal testing: Please contact the laboratory for instructions.

TURNAROUND TIME:     8 working days

CPT CODES: